Título provisional de la tesis

Unveiling the function of the LSD1 complexes during adventitious root formation

Resumen

Plants have a remarkable regenerative capacity, of which their ability to form adventitious roots (ARs) (postembryonic roots formed de novo from non-root tissues) is a good example. With the aim of initiating the genetic dissection of wound-induced AR formation in tomato, a screening of more than 3000 lines was performed, with the more adventitious roots1 (mars1) mutants being the most interesting mutants found. mars1 is affected in a gene encoding a conserved LYSINE-SPECIFIC DEMETHYLASE 1, LSD1, an epigenetic enzyme that specifically demethylates H3K4me1/me2 or H3K9me1/me2 marks in animals and can function as a transcriptional repressor or activator. The goals of this project are: (1) obtain the complete transcriptome of AR primordia in wild-type and mars1 seedlings at a single cell resolution, (2) characterize the epigenetic signatures of LSD1-regulated target genomic regions, (3) confirm the functional relevance of at least three LSD1-regulated targets during AR formation, (4) identify conserved LSD1-like interactors by a TAP-tagged approach, and (5) confirm the functional relevance of some of these core LSD1 interactors by loss-of-function analyses.

Director/a: José Manuel Pérez Pérez

Publicaciones derivadas de la tesis:

Código ORCID: 0009-0003-1508-4294